Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (2024)

+353 61 371 725 info@celignis.com

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (1)

  • Feedstocks Analysed at Celignis
    Spent Mushroom Compost

Background on Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (3)


Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (4)


Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (5)

Spent mushroom compost (SMC) is the substrate remaining after mushroom production, with approximately 5 kg of SMC produced for each kg of mushrooms. Mushroom compost is a mixture of 60 to 70% straw, 28 to 34% poultry litter, and 2 to 4.5% gypsum. It is made in a series of stages, termed phases. In the first phase the components (e.g. straw, litter, gypsum) are mixed and then placed in long windrows for a period of up to 2 weeks with the resulting product being termed Phase I compost. The second phase takes up to 18 days and takes place indoors in plastic tunnels that allow for the environment to be controlled so that any unwanted organisms or diseases in the compost can be controlled. Once the compost is of a quality suitable for mushroom production the compost is mixed with spawn, a monoculture of mushroom mycelium on grain. This compost is termed Phase II. Phase III involves the spawning and growth of the mycelium and takes place under controlled conditions. It is considered complete when the mycelia have fully colonised the compost.

Mushroom producers either receive Phase II or Phase III composts. Once the compost is fully colonised mushroom production involves placing a casing layer of peat on top of the compost. This layer promotes the formation of promordia; mushroom pins. Approximately three weeks after this point the first crop (first flush) of mushrooms can be harvested. The compost can then be rewet allowing for the harvesting of subsequent flushes at approximately 7 day intervals. Typically up to three flushes are harvested from each compost shipment. The remaining material is known as SMC and can sometimes be sterilised (cooked out) by heating for 12 hours at 70 degrees Celsius.

The overall composition of SMC will vary according to the time of year, the amount of peat casing put on by the grower, the compost manufacturers, and the amount of water added to the mushroom by the grower. The chemical composition of the ultimate spent material will be significantly different from the composite of the materials that make up the mushroom compost and casing layer, however, due to the effects of the composting process and mushroom growth.


Analysis of Spent Mushroom Compost at Celignis

Celignis Analytical can determine the following properties of Spent Mushroom Compost samples:

Lignocellulosic Properties of Spent Mushroom Compost

Cellulose Content of Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (6)

Celignis founder Daniel Hayes has extensive experience in the collection, preparation, and chemical/infrared analysis of mushroom composts and spent mushroom composts. He has carried out a research project, funded by the Irish Environmental Protection Agency, that involved the analysis of a number of samples. These samples covered the various stages of production of mushroom compost as well as spent mushroom composts produced in different greenhouses under different numbers of flushes.

Typically the cellulose content of spent mushroom compost is higher than the hemicellulose content but lower than the lignin content.

Click here to see the Celignis Analysis Packages that determine Cellulose Content

Request a QuoteCellulose Content

Hemicellulose Content of Spent Mushroom Compost

Xylose is the principal hemicellulose sugar in most spent mushroom composts. However the ratios of the different sugars can vary substantially according to the stage of mushroom production. Therefore, specific analysis of the sample in question is recommended.

Click here to see the Celignis Analysis Packages that determine Hemicellulose Content

Request a QuoteHemicellulose Content

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (7)

Lignin Content of Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (8)

The lignin content of spent mushroom composts can be significant and can also vary greatly depending on the biomass that was used to make the compost and on the conditions used for mushroom growth.

Click here to see the Celignis Analysis Packages that determine Lignin Content

Request a QuoteLignin Content

Starch Content of Spent Mushroom Compost

The starch content of spent mushroom compost is typically low as starch will have been utilised by the mushrooms.

Click here to see the Celignis Analysis Packages that determine Starch Content

Request a QuoteStarch Content

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (9)

Uronic Acid Content of Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (10)

Uronic acids are present in the straws that are typically used to generate mushroom compost, however we are not aware of any studies to date on the fate of these uronic acids during the composting process and post mushroom production.

Click here to see the Celignis Analysis Packages that determine Uronic Acid Content

Request a QuoteUronic Acid Content

Enzymatic Hydrolysis of Spent Mushroom Compost

We can undertake tests involving the enzymatic hydrolysis of Spent Mushroom Compost. In these experiments we can either use a commercial enzyme mix or you can supply your own enzymes. We also offer analysis packages that compare the enzymatic hydrolysis of a pre-treated sample with that of the native original material.

Click here to see the Celignis Analysis Packages that determine Enzymatic Hydrolysis

Request a QuoteEnzymatic Hydrolysis

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (11)

Bioenergy Properties of Spent Mushroom Compost

Ash Content of Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (12)

Ash content of spent mushroom compost can be high and can also vary significantly.

Click here to see the Celignis Analysis Packages that determine Ash Content

Request a QuoteAsh Content

Heating (Calorific) Value of Spent Mushroom Compost

The heating value of spent mushroom compost is often quite low due to the high moisture and ash contents of the feedstock. Despite this, however, there has been commercial interest in the utilisation of spent mushroom compost in energy/power generation facilities. This is primarily due to its very low cost. Indeed spent mushroom compost can often be obtained for a gate fee.

Click here to see the Celignis Analysis Packages that determine Heating (Calorific) Value

Request a QuoteHeating (Calorific) Value

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (13)

Ash Melting Behaviour of Spent Mushroom Compost

Ash melting, also known as ash fusion and ash softening, can lead to slagging, fouling and corrosion in boilers which may reduce conversion efficiency. We can determine the ash melting behaviour of Spent Mushroom Compost using our Carbolite CAF G5 BIO ash melting furnace. It can record the following temperatures:

Ash Shrinkage Starting Temperature (SST) - This occurs when the area of the test piece of Spent Mushroom Compost ash falls below 95% of the original test piece area.

Ash Deformation Temperature (DT) - The temperature at which the first signs of rounding of the edges of the test piece occurs due to melting.

Ash Hemisphere Temperature (HT) - When the test piece of Spent Mushroom Compost ash forms a hemisphere (i.e. the height becomes equal to half the base diameter).

Ash Flow Temperature (FT) - The temperature at which the Spent Mushroom Compost ash is spread out over the supporting tile in a layer, the height of which is half of the test piece at the hemisphere temperature.

Click here to see the Celignis Analysis Packages that determine Ash Melting Behaviour

Request a QuoteAsh Melting Behaviour

Major and Minor Elements in Spent Mushroom Compost

Examples of major elements that may be present in Spent Mushroom Compost include potassium and sodium which are present in biomass ash in the forms of oxides. These can lead to fouling, ash deposition in the convective section of the boiler. Alkali chlorides can also lead to slagging, the fusion and sintering of ash particles which can lead to deposits on boiler tubes and walls.

We can also determine the levels of 13 different minor elements (such as arsenic, copper, and zinc) that may be present in Spent Mushroom Compost.

Click here to see the Celignis Analysis Packages that determine Major and Minor Elements

Request a QuoteMajor and Minor Elements

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (15)

Analysis of Spent Mushroom Compost for Anaerobic Digestion

Biomethane potential (BMP) of Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (16)

Given that spent mushroom compost has high ash and lignin contents, it is not considered to be a good feedstock for anaerobic digestion. The matter is made worse by the low contents of labile sugars (e.g. water soluble carbohydrates) and relatively small amounts of cellulose and hemicellulose in the feedstock. Hence, even when expressed on a non-lignin volatile solids basis, the biochemical methane potential (BMP) is likely to be low.

Click here to see the Celignis Analysis Packages that determine BMP

Request a QuoteBMP

Physical Properties of Spent Mushroom Compost

Bulk Density of Spent Mushroom Compost

At Celignis we can determine the bulk density of biomass samples, including Spent Mushroom Compost, according to ISO standard 17828 (2015). This method requires the biomass to be in an appropriate form (chips or powder) for density determination.

Click here to see the Celignis Analysis Packages that determine Bulk Density

Request a QuoteBulk Density

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (17)

Particle Size of Spent Mushroom Compost

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (18)

Given that spent mushroom compost has high ash and lignin contents, it is not considered to be a good feedstock for anaerobic digestion. The matter is made worse by the low contents of labile sugars (e.g. water soluble carbohydrates) and relatively small amounts of cellulose and hemicellulose in the feedstock. Hence, even when expressed on a non-lignin volatile solids basis, the biochemical methane potential (BMP) is likely to be low.

Click here to see the Celignis Analysis Packages that determine Particle Size

Request a QuoteParticle Size

Publications on Spent Mushroom Compost By The Celignis Team

Hayes, D. J. M. (2011) Analysis of Lignocellulosic Feedstocks for Biorefineries with a Focus on The Development of Near Infrared Spectroscopy as a Primary Analytical Tool, PhD Thesis832 pages (over 2 volumes)

Download

The processing of lignocellulosic materials in modern biorefineries will allow for theproduction of transport fuels and platform chemicals that could replace petroleum-derivedproducts. However, there is a critical lack of relevant detailed compositional informationregarding feedstocks relevant to Ireland and Irish conditions. This research has involved thecollection, preparation, and the analysis, with a high level of precision and accuracy, of alarge number of biomass samples from the waste and agricultural sectors. Not all of thewaste materials analysed are considered suitable for biorefining; for example the total sugarcontents of spent mushroom composts are too low. However, the waste paper/cardboardthat is currently exported from Ireland has a chemical composition that could result in highbiorefinery yields and so could make a significant contribution to Ireland’s biofuel demands.

Miscanthus was focussed on as a major agricultural feedstock. A large number of plantshave been sampled over the course of the harvest window (October to April) from severalsites. These have been separated into their anatomical fractions and analysed. This hasallowed observations to be made regarding the compositional trends observed withinplants, between plants, and between harvest dates. Projections are made regarding theextents to which potential chemical yields may vary. For the DIBANET hydrolysis processthat is being developed at the University of Limerick, per hectare yields of levulinic acid fromMiscanthus could be 20% greater when harvested early compared with a late harvest.

The wet-chemical analysis of biomass is time-consuming. Near infrared spectroscopy (NIRS)has been developed as a rapid primary analytical tool with separate quantitative modelsdeveloped for the important constituents of Miscanthus, peat, and (Australian) sugarcanebagasse. The work has demonstrated that accurate models are possible, not only for dryhom*ogenous samples, but also for wet heterogeneous samples. For glucose (cellulose) theroot mean square error of prediction (RMSEP) for wet samples is 1.24% and the R2 for thevalidation set () is 0.931. High accuracies are even possible for minor analytes; e.g. forthe rhamnose content of wet Miscanthus samples the RMSEP is 0.03% and theis 0.845.Accurate models have also been developed for pre-treated Miscanthus samples and arediscussed. In addition, qualitative models have been developed. These allow for samples tobe discriminated for on the basis of plant fraction, plant variety (giganteus/non-giganteus),harvest-period (early/late), and stand-age (one-year/older).

Quantitative NIRS models have also been developed for peat, although the heterogeneity ofthis feedstock means that the accuracies tend to be lower than for Miscanthus. Thedevelopment of models for sugarcane bagasse has been hindered, in some cases, by thelimited chemical variability between the samples in the calibration set. Good models arepossible for the glucose and total sugars content, but the accuracy of other models ispoorer. NIRS spectra of Brazilian bagasse samples have been projected onto these models,and onto those developed for Miscanthus, and the Miscanthus models appear to provide abetter fit than the Australian bagasse models.


Examples of Other Feedstocks Analysed at Celignis

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (19)

See our pitches for the 2024 topics.


Special Offer

Mar 30th 2024

CBE-JU 2024 Call Opens - See our Pitches for Proposals

Click here to view our pitches for involvement in proposals for the 2024 research topics of the Circular Bioeconomy Europe Joint Undertaking (CBE-JU).

View Pitches

Analysis Packages

Lignocellulose Composition

P19 : Deluxe Lignocellulose Package
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.

P11 : NIR Prediction Package
Ligno. Sugars, Lignin, Ethanol-Soluble Extractives, Ash

P20 : Lignin S/G Ratio
Ratio of syringyl (S) and guaiacyl (G) lignin units

Enzyme Activity and Enzymatic Hydrolysis

P123 : Composition of Residue from Enzymatic Hydrolysis
As P9 but on the solid residue after enzymatic hydrolysis.

Analysis of Biomass Process Liquids

Seaweed (Macroalgae) Analysis

Anaerobic Digestion

Biochar Production and Analysis

Biochar Production

P350 : Biochar Production
At various temperatures and residence times

Biochar Physical Properties

P368 : Pore Size Distribution Ultimate
Using nitrogen and carbon dioxide

Biochar Thermal Properties

P394 : Biochar Thermal Properties Ultimate
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture

Biochar Soil Amendment Properties

P396 : Biochar Soil Amendment Deluxe
Includes P381, P382, P384, P385 and P386

P397 : Biochar Soil Amendment Ultimate
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)

Biochar Complete Analysis

P399 : Biochar Complete Evaluation Package
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)

Combustion Properties

P50 : Ultimate Combustion Package
Includes P36, P40 and P42

Fibre Analyses

News

Jan 24th 2024

€1.5m Funding Success in CBE-JU
Celignis is a Partner in 3 Successful Proposals for EU Funding

We are pleased to announce that three of the proposals involving Celignis, submitted to the CBE-JU programme for funding collaborative biomass research in Europe, were successful. These projects will provide an additional funding of €1.5m to Celignis and build on our achievements in other CBE and EU projects. In particular, the projects are all at enhanced TRLs (6/7) and will use our existing Celignis Bioprocess infrastructure and will also fund further development of our bioprocessing capacities and the Bioprocess Development Services we offer our clients.

Details on the funded projects are provided below:

BIONEER - This project was funded under CBE-JU topic IA-06 and focuses on the TRL 6/7 production of biobased platform chemicals. Celignis's activities in the project focus on scaling up the work undertaken in our ongoing

Read...

Jan 23rd 2024

Celignis to Exhibit and Present at Major Biochar Event
The 2024 North American Biochar Conference will take place in Sacramento, California, on Feb 12-15

On Feb 12-15 we'll be exhibiting at the 2024 North American Biochar Conference, taking place at the SAFE Credit Union Convention Centre in Sacramento, California.

We're looking forward to interacting with the 1000+ expected attendees, outlining our extensive range of analytical and application testing services for biochar.

Celignis CIO Lalitha Gottumukkala will also be a member of the expert panel focused on developing improved laboratory methods for biochar characterisation.

Click here to register for the event.

Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (20)

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Jan 22nd 2024

Celignis Attending BIC Event on Feb 8
This Networking Event Will Involve Discussions on Collaborations for Proposals to the 2024 CBE-JU Topics

The Circular Bioeconomy Europe Joint Undertaking (CBE-JU) is an organisation that funds biomass research in Europe at various Technology Readiness Levels (TRLs). Since 2016 Celignis has been an active participant in a number of projects funded by the CBE-JU.

The Biobased Industries Consortium (BIC) is the steering committee that helps to steer the focus of research for the CBE-JU programme. In 2023 Celignis joined the BIC as a Full Industry Member and participated in several proposals submitted for different research topics in the CBE-JU's 2023 Work Programme.

On Feb 8th Celignis's Dan Hayes, Lalitha Gottumukkala, and Oscar Bedzo will be attending a BIC networking event in Brussels where we will discuss potential collaborations in the research programme topics recently announced for 2024.

Read...

Jan 19th 2024

We're Hiring - Business Administration & Client Relationship Manager
This position will involve working closely with senior management, fostering existing and new client relationships.

Situated in Limerick, Ireland, Celignis currently operates at two centres, Celignis Analytical and Celignis Bioprocess, actively engaging in a variety of private and public bioeconomy projects. As we continue to expand, we're looking to strengthen our team of 14 with a Business Administration and Client Relationship Manager who can bring a blend of enthusiasm and expertise.

This position will involve working closely with senior management, fostering existing and new client relationships, and ensuring successful delivery of our services, playing a key role in our ongoing growth and success.

Click here for more details about the position.

Read...

Apr 30th 2023

Celignis to Sponsor and Present at Major Biochar Event
The event takes place on May 3rd at Carrick-on-Shannon

We are pleased to announce that, on May 3rd, Celignis will be presenting and exhibiting at the National Biochar and Carbon Products Conference 2023, which is taking place in Carrick-on-Shannon in County Leitrm, Ireland.

This conference is being organised under the auspices of the Interreg Northwest Europe-funded THREE C Project, entitled 'Creating and sustaining Charcoal value chains to promote a Circular Carbon economy in NWE Europe'.

The conference will highlight both Irish stakeholders who are currently working in the biochar and carbon products sector, but also partners from the THREE C project (covering Netherlands, Luxembourg, Germany, Belgium, France and Wales, as well as Ireland) who have interesting stories and products to share.

Read...

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Analysis of =$DATAADAPTER['name']?>, Cellulose Content of =$DATAADAPTER['name']?>, Lignin Content of =$DATAADAPTER['name']?> (2024)
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