Bulk substrate (2024)

Bulk substrates are moderately nutritious materials used in mass mushroom cultivation. Bulk substrates are often used in conjunction with a pre-colonized grain spawn which is used to inoculate the bulk substrate.

Contents

  • 1 Pasteurization vs sterilization
  • 2 Common bulk substrates
    • 2.1 Manure/Compost
    • 2.2 Coconut coir
    • 2.3 Straw
    • 2.4 Hardwood
  • 3 Supplements
    • 3.1 Gypsum
    • 3.2 Coffee grounds
    • 3.3 Chicken manure
    • 3.4 Vermiculite
    • 3.5 Worm castings
  • 4 Guides
    • 4.1 Pasteurization
    • 4.2 Complete growing guides

Pasteurization vs sterilization[]

When moderately nutritious bulk substrates are pasteurized at 140-175°F (60-80°C), some beneficial micro organisms, mainly bacteria, stay alive, inhabit the substrate and guard it against other, more aggressive micro organisms. This resistance to contamination is the reason bulk substrate can be inoculated with spawn in open spaces without taking special sterile precautions. Even with these micro organisms, mycelium will still able to grow on this substrate though.

If you sterilize the bulk substrate it becomes as nearly as susceptible to contaminants as highly nutritious spawn substrates like rye berries and brown rice. This means that you would have to inoculate the big amount of bulk substrate in sterile conditions, such as in front of a laminar flow hood and the substrate must stay in sterile conditions until it is fully colonized. This is not practical for large amounts of substrates.

The exception to this rule is when large, sterile spawn bags are used, but in that case, the cultivator is better off using entirely highly nutritious spawn substrate instead of the moderately nutritious bulk substrate.

Common bulk substrates[]

Manure/Compost[]

Manure is the aged, dried excrement of horses, cows, elephants, etc. It is one of the most effective bulk substrates for dung loving species like psilocybe cubensis, panaeolus cyanescens and agaricus bisporus (Portobello). It is usually cheap or free if it can be located. Many serious cultivators will compost their manure with other additives in order to produce an optimal substrate.

Coconut coir[]

Coconut coir is the shredded fiber of coconut husks. It holds many times its weight in water but does not decostores and hardware stores sell it in compressed bales & pet stores sell it in dried bricks. Coir is very low in nitrogen, so coffee grounds or blood meal are also common additives.

Straw[]

Wheat straw is commonly available in farm/fleet and crafts store. It is extremely inexpensive and relatively easy to work with. Due to its high nitrogen content and porous texture, straw makes an excellent bulk substrate for wood loving mushrooms and pleurotus ostreatus (Oysters).

Hardwood[]

Some mushrooms, such as lentinula edodes(Shiitake) and psilocybe cyanescens, thrive on hardwood based bulk substrates. Wood holds water well and provides a nutritious growing medium. Softwoods (needle bearing trees) are not suitable as substrates since they contain natural fungicides, but the majority of softwoods used for construction will have those chemicals bleached out.

Supplements[]

Gypsum[]

Gypsum is used to improve the structure of the bulk substrate and to act as a pH buffer. It is usually added at 5-10% by volume.

Coffee grounds[]

Used coffee grounds are a common additive to nitrogen deficient substrates (like coconut coir). Some commercial growers even grow purely on a coffee based substrate. When supplementing another substrate, coffee grounds are often added at 15-20% by volume.

Chicken manure[]

Chicken manure is not suitable as a bulk substrate due to its excessive nitrogen content and muddy consistency, but it is very effective as a supplement to other bulk substrates.

Vermiculite[]

Vermiculite is sometimes used in bulk substrates in order to increase the amount of water retention. It usually added at 10-50% by volume.

Worm castings[]

Although worm casting (aka worm poop) can be used as a bulk substrate by itself, it is most commonly used as a supplement to another bulk substrate since it turns into mud when it is hydrated.

Guides[]

Pasteurization[]

  • Pasteurization in mason jars

Mason jar pasteurization is a good method for pasteurizing up to seven quarts at a time. The advantages to using this method are more precise control of moisture(hydrate the substrate to a little more than field capacity as some moisture will be lost in the process) and temperature control, as you can measure the temperature in each jar.Using a canning pot with the suspension racks, fill seven quart jars with your substrate mix. Then fill the pot until it is a quarter full, and put the lid on. I do not mind water dripping back into the jars as it replaces some of the lost moisture. Heat to 165-180(I prefer 180) for 90 minutes. Let cool until the substrate mix is BELOW 80 DEGREES before spawning your jars.

Always spawn to the substrate as quickly as is allowed by the temperature of the substrate, as it is a race between your mycellieum and whatever contaminants remain in the substrate.

Follow the same proceedures as above, but you are using a pillowcase full of substrate. This method allows for a larger bulk to to be processed, but is at a disadvantage when it comes to moisture regulation. Make sure to check your subsrate's moisture content after using this method. Dry weight vs wet weight is the best way, but the squeeze test is a good rule of thumb as well. To use the the squeeze test, as you load the sub, squeeze every third handfull. The sub should give up a short (less than one second) sream of water, followed by drops, tapering off to nothing. This entire water loss should take no more than five seconds until little or no water can be squeezed out of the substrate. Pressure applied to the squeeze should be the equivilant of a firm handshake.

Complete growing guides[]

Bulk substrate (2024)

FAQs

What is a bulk substrate? ›

Bulk substrates are moderately nutritious materials used in mass mushroom cultivation. Bulk substrates are often used in conjunction with a pre-colonized grain spawn which is used to inoculate the bulk substrate.

Do you need to sterilize bulk substrate? ›

You'll need to sterilize high nutrient substrates or substrates with high levels of supplementation like manure, soy hulls, grains and master's mix. But less nutritious substrates like straw, sugarcane bagasse, coco coir, hardwood sawdust and cardboard only need pasteurization.

How long can bulk substrate sit? ›

Store your 50/50 substrate in a cool and dry location. 50/50 substrate can keep for up to 4 months in these conditions. There will be no need to rehydrated or sterilize the products a second time. If you need to store the product longer it can be refrigerated for up to 6 months and frozen for up to 1 year.

Should I mist my bulk substrate? ›

If you plan on fruiting the kit in the bag, you will want to open the bag to give the substrate some fresh air once it is fully colonized. Then you may need to mist the substrate with clean or distilled water if it appears to be drying out.

When to fruit bulk substrate? ›

Fruiting Bulk Substrate

After the substrate is totally colonized it is ready to be put in fruiting conditions. This requires fruiting chambers suited for bulk growing such as a monotub or double tub.

How wet should my bulk substrate be? ›

Your substrate should be slightly acidic, with a PH between 5 and 6.5. (Some mushrooms, such as oyster mushrooms, can withstand a PH of up to 8.) A minimum moisture content of 50-70% is essential for your substrate.

How long to soak bulk substrate? ›

Hot water pasteurization works well for small-scale home cultivators but gets expensive when pasteurizing bulk substrate. It involves submerging your substrate in hot water between 149 and 167°F (65 and 75°C) for one to two hours.

What happens if you don't sterilize mushroom substrate? ›

Mushroom substrates are the perfect breeding ground for unwanted microbes. If not properly treated through pasteurization or sterilization, contamination will lead to wasted crops.

How long does it take to colonize bulk substrate? ›

It will take approximately 5-10 days for the substrate to completely colonize with mycelium (you will be able to tell if it has completed colonization when the substrate has turned completely white).

What temperature do you pasteurize bulk substrate? ›

Multiple methods exist to "clean" or "pasteurize" your substrate before integrating it with our GIY material. The common objective of these methods is to reach a temperature of 140-160° F (60-71° C) for 2-4 hours, effectively eliminating competing organisms.

What happens if substrate is too wet? ›

However, if you have too much moisture, your mycelium will also be stunted, as it will suffocate. With too much moisture, your substrate will also have a higher risk of contamination from other organisms.

What is the bulk substrate for cubensis? ›

The Psilocybe Cubensis family is known to grow well on a standard bulk substrate recipe – a mixture of coco-coir / vermiculite / gypsum (CVG).

Why use bulk substrate? ›

One of the key elements of successful mushroom cultivation is the use of a suitable bulk substrate that your mushroom mycelia like. A bulk substrate is the material on which the mushroom mycelium (the vegetative part of the fungus) grows. It provides both a food source and a water source for your mushrooms to grow.

How deep should substrate be in a Monotub? ›

The amount of bulk substrate and spawn you use will be dependent on the size of your monotub. You want to have 3 - 5 inches of substrate.

What are the three types of substrate? ›

Substrates can be classified according to their properties and composition. From these properties we can define what type of substrate works well for each type of plant. Depending on their properties, substrates can be organic and mineral or inorganic.

What is an example of a substrate? ›

Carbohydrates like glucose, sucrose, starch act substrates for enzymes like salivary amylase, maltase. Amino acids, peptides, proteins act as substrates for enzymes trypsin, chymotrypsin, etc. These proteins are present in grams, the meat we eat.

What does it mean when a drug is a substrate? ›

Substrates are drugs that bind to the active site of an enzyme and are transformed into metabolites while being present in this active site. The biotransformation process of a drug may involve multiple enzymes leading to various metabolites; each metabolic route relies on specific characteristics.

What is the definition of a substrate? ›

In biology, a substrate can be the surface on which an organism (eg: plant, fungus, or animal) lives or the substance on which an enzyme can act. In geology, a substrate is a rock or sediment surface where chemical and biological processes occur.

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