Let’s talk about STERILIZATION - FloCube (2024)

Let’s talk about STERILIZATION - FloCube (1)

All microbiology textbooks state that wet heat sterilization requires a temperature of 250°F for 20 minutes. Therefore, before inoculation, supplemented sawdust fruiting blocks and mushroom grain spawn must be sterilized entirely. This requires exposing the substrate or grain to high pressure and temperature for a long duration to ensure the core temperature is reached and proper sterilization is achieved.

The best method is to use a stovetop pressure canner large enough to contain the material and produce 15 PSI. The Presto 23L is the go-to for most of us. Sterilization time for supplemented sawdust fruiting blocks should be 2.5 hours, although grain spawn can typically be sterilized in 120 minutes. I prefer to sterilize my grain jars for 120 minutes and bags for 180 minutes.

SubstrateSterilization

When sterilizing substrates, grains, and agar mixtures, search for a pressure cooker that can hold a pressure of 15 PSI, which allows for a steam temperature of 250°F. Hot enough to eliminate any competing mold or bacteria, giving the desired mushroom species the best chance of survival.

Can I use lower pressure?

Is 12 PSI sufficient?

Is it possible to get away with 5 PSI?

If the pressure is lower, complete sterilization can still be achieved by increasing the sterilization time.

Let’s talk about STERILIZATION - FloCube (3)

Suggested Sterilization Times @ 15 PSI

  • Liquid Agar 30 minutes
  • Quart Size Grain Jars 120 minutes
  • 5lb Grain Bags 180 minutes
  • 5lb Sawdust Block 180 minutes

Hope this helps.Leave a comment and let us know what works for you!

Let’s talk about STERILIZATION - FloCube (2024)

FAQs

Let’s talk about STERILIZATION - FloCube? ›

When sterilizing substrates, grains, and agar mixtures, search for a pressure cooker that can hold a pressure of 15 PSI, which allows for a steam temperature of 250°F. Hot enough to eliminate any competing mold or bacteria, giving the desired mushroom species the best chance of survival.

Why was sterilization controversial? ›

Racism and eugenic beliefs perpetuated coerced and involuntary sterilization against women of color, especially African Americans, Latinas, and Native Americans. These groups of women were often targeted as a way to reduce their population and limit their childbearing abilities.

How long do you sterilize grain spawn? ›

4 Steps to Making Grain Spawn

Step 1: Rinse and clean the grains, soak in water for 12-24 hours. Step 2: Cook the grains for 15-20 minutes so that they absorb water. Step 3: Drain and dry excess moisture from the outside of the grains. Step 4: Load grain into mason jars and sterilize for 90 minutes at 15 PSI.

What is so great about sterilization? ›

Sterilization is VERY effective.

Tubal ligation is permanent and one of the most effective kinds of birth control out there — more than 99% effective at preventing pregnancy. Tubal ligation works so well because it's permanent, and there's almost no way you can mess it up or use it the wrong way.

What are the basics of sterilization? ›

Sterilization, which is any process, physical or chemical, that destroys all forms of life, it is used especially to destroy microorganisms, spores, and viruses. Precisely defined, sterilization is the complete destruction of all microorganisms by a suitable chemical agent or by heat, either wet steam.

Did doctors sterilize Native Americans? ›

In the 1970s, doctors in the United States sterilized an estimated 25 to 42 percent of Native American women of childbearing age, some as young as 15. Even the lower estimate—one quarter of Native women—is a whopping statistic.

When did they stop sterilizing people? ›

1981. 1981 is commonly listed as the year in which Oregon performed the last legal forced sterilization in U.S. history.

How long will sterilized spawn bags last? ›

How long are they good for? A: It is best to use your kits or grain bags within 30 days of receiving them. We have performed quality control tests on our bags time and time again and provided they have not been opened and exposed to outside air, they can keep at room temperature for six months or longer.

What temperature do you sterilize grains? ›

When sterilizing substrates, grains, and agar mixtures, search for a pressure cooker that can hold a pressure of 15 PSI, which allows for a steam temperature of 250°F.

How to sterilize grain spawn without a pressure cooker? ›

Simply fill a large pot with water, place the jars inside, and bring the water to a boil. Once the water is boiling, lower the heat and let the jars simmer for about 60-90 minutes. Keep an eye on the water level to make sure it doesn't boil dry. After sterilization, allow the jars to cool before inoculating.

What is sterilization regret? ›

Sterilization regret was defined as someone who either underwent sterilization reversal or who definitely wanted sterilization reversal. Multivariable logistic regression models were used to assess associations with sterilization regret.

Does bleach sterilize? ›

Bleach is a strong and effective disinfectant – its active ingredient sodium hypochlorite is effective in killing bacteria, fungi and viruses, including influenza virus – but it is easily inactivated by organic material. Diluted household bleach disinfects within 10–60 minutes contact time (see Table G.

Why is sterilization a problem? ›

These coercive and abusive population control policies impacted people around the world in different ways, and continue to have social, health, and political consequences, one of which is lasting mistrust in current family planning initiatives by populations who were subjected to coercive policies like forced ...

Which action destroys all viruses and spores? ›

Sterilization describes a process that destroys or eliminates all forms of microbial life and is carried out in health-care facilities by physical or chemical methods.

What are the 7 steps of sterilization? ›

Contents
  • 1 Applications. 1.1 Foods. 1.2 Medicine and surgery. ...
  • 2 Quantification.
  • 3 Heat. 3.1 Steam. 3.2 Dry. ...
  • 4 Chemical sterilization. 4.1 Ethylene oxide. 4.2 Nitrogen dioxide. ...
  • 5 Radiation sterilization. 5.1 Non-ionizing radiation sterilization. ...
  • 6 Sterile filtration.
  • 7 Preservation of sterility.
  • 8 See also.

What is the sterilization rule? ›

The general acceptance criteria include internal temperature greater than 121 °C; external temperature greater than 115 °C; and pressure greater than 1.1 atm. These figures must be maintained for the whole of the sterilization period, and no drop must be seen.

What are the major problems with sterilization? ›

Sterilization: Long-Term Issues
  • REGRET AFTER STERILIZATION. ...
  • STERILIZATION FAILURE AND ECTOPIC PREGNANCY. ...
  • MENSTRUAL CYCLE CHANGES. ...
  • HYSTERECTOMY. ...
  • POSTABLATION TUBAL STERILIZATION SYNDROME. ...
  • BREAST CANCER, ENDOMETRIAL CANCER, AND BONE MINERAL DENSITY. ...
  • OVARIAN CANCER. ...
  • SEXUALLY TRANSMITTED INFECTIONS AND PELVIC INFLAMMATORY DISEASE.

What arguments were used to end forced sterilization? ›

v. Bolivia before the Inter-American Court of Human Rights. We urged the Court to view forced sterilization as a multiple rights violation, to hold that it violates the human right to dignity, and to include mandatory training for health care professionals among its ordered reparations in that case.

What is the moral issue of sterilization? ›

Coercive or forcible sterilization practices are unethical and should never be performed. Ethical sterilization care requires access to sterilization for women who request it, without undue barriers.

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